Yes. The European TCO authorisation regime does not differentiate between scheduled and non-scheduled commercial air transport operations. All commercial air transport air operators need to apply for a TCO authorisation.
For air ambulance flights and other urgent flights performed in the public interest and where the urgency of the mission justifies bypassing the regular TCO assessment process (e.g. humanitarian missions or disaster relief operations), please consult the section for “One-off” notification flights.